By Claudio Ortolani
Flow Cytometry of Hematological Malignancies includes an array of graphical outputs produced by means of the procedure within the examine of the main (and the least) universal ailments. the pictures integrated let you evaluate your personal effects with a 3rd get together reference trend. there's a exact description of the most leukocyte antigens, including an outline in their distribution among basic and irregular blood cells. The ebook additionally offers a entire description of the phenotype of each neoplastic blood disorder recorded within the WHO class procedure, together with the entire directions had to know and classify even the least universal entity.
Designed to be functional, the publication is ideal for speedy session and is split into major sections. part I bargains with the direct item of immunophenotyping, and part II offers with the last word aim of the research. greater than 50 antigens are coated and each antigen is handled in 3 major elements: normal gains, cytometric positive aspects and sensible tricks.
This authoritative and state of the art reference should be beneficial for clinicians at once considering the prognosis and research of hematological illnesses, together with hematologists, hematopathologists, oncologists, pathologists and technicians operating in diagnostic laboratories.Content:
Chapter 1 Antigens (pages 1–157):
Chapter 2 illnesses (pages 160–300):
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Extra info for Flow Cytometry of Hematological Malignancies
C) anaplastic large cell lymphoma (ALCL). 29 Bivariate analysis carried out for CD13 and CD16 on the normally maturing bone marrow myeloid component of two normal donors. The analysis produces a typical graphic pattern consisting of a V-shaped cluster. CD14 Antigen General features CD14 is a 55 kD glycoprotein encoded by a gene situated on chromosome 5 . e. the lipopolysaccharide-binding protein (LBP) . CD14 is expressed brightly on monocytes, while on promonocytes it is missing or is expressed at a lower intensity .
CD13 expression has been reported on synovial ﬂuid T cells from patients with arthritis  and on lymphocytes inﬁltrating renal cell carcinoma . Cytometric features The staining of normal peripheral blood with an anti-CD13 monoclonal antibody generates a bimodal positive histogram with a ﬁrst mode related to a value of 17Ϯ6 E03 ABC for polymorphonuclear granulocytes, and a second mode related to a value of 21Ϯ8 E03 ABC for monocytes  (Fig. 26). 36 During myeloid maturation, CD13 appears before CD33 on CD34ϩ myeloid precursors; consequently normal myeloid precursors displaying CD13ϩ CD33– CD34ϩ CD90ϩ CD117ϩ and CD133ϩ phenotype can be found in cord blood and bone marrow .
B) mantle cell lymphoma (MCL). (C) anaplastic large cell lymphoma (ALCL). 29 Bivariate analysis carried out for CD13 and CD16 on the normally maturing bone marrow myeloid component of two normal donors. The analysis produces a typical graphic pattern consisting of a V-shaped cluster. CD14 Antigen General features CD14 is a 55 kD glycoprotein encoded by a gene situated on chromosome 5 . e. the lipopolysaccharide-binding protein (LBP) . CD14 is expressed brightly on monocytes, while on promonocytes it is missing or is expressed at a lower intensity .