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Additional resources for Experimental Hematology Today 1978
USA), 68:2734, 1971. , and Keighley, G. Two hereditary mouse anemias (SId/SI d and W/WV) deficient in response to erythropoietin. Ann N. Y. Acad. Sci .. 149:475, 1968. Byron, J. Molecular basis for the triggering of hematopoietic stem cells into DNA synthesis. , Hemopoiesis in Culture. S. Government Printing Office, 1974, p. 91. R. Decreased neutrophils and megakaryocytes in anemic mice by donor cells. Transplantation. 22:42, 1969. , and Stohlman, F. Abnormalities of megakarocytes in W/Wv mice.
Restoration of normal erythropoiesis in these mice require an interaction between the stem cell and the TSRC (11). It, therefore, became of interest to see if the defective stem In Vivo Thymosin Therapy Thymosin was given to W/Wv recipient mice for 6 weeks following a marrow graft of 107 TSRCdepleted cells. 5 ml. For spleen colony assay, 105 TSRC-depleted cells were given followed by three doses of thymosin on alternate days (700 JLg/dose) and the W/Wv mice were sacrificed 7 days after marrow grafting.
Z E ... 'co a 50 't: 25 '0 gc o 5 Z -e. u c 2 c. 1 :2 1:4 1:8 1 :16 1:64 1:32 1: 128 Serial dilutions FIGURE 4·1. Number of surface spleen colonies formed from 5 x 10· marrow cells treated with serial dilutions of RAMBS-r in the presence of complement. The data are presented as means ± standard error (SEM). There were nine to 10 assay mice in each RAMBS-r group. , prior to the addition of complement, are indicated. The number of spleen colonies formed after treatment with CRS-r (cross-hatched histogram) is the mean of three separate assays (nine to 10 mice/assay) using dilutions at 1:2, 1:4, and 1:8 during the primary incubation.