Download Developmental Hematopoiesis: Methods and Protocols by Margaret H. Baron PDF

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By Margaret H. Baron

The 1st selection of ways to learn embryonic and fetal hematopoiesis in either invertebrate (fruit flies) and vertebrate (frog, fish, mouse, chick, and human) organisms. those cutting-edge options diversity from the genetic, molecular, and mobile, to phone and embryo explant tradition and entire animals, together with in vivo imaging. Bioinformatic and useful genomic techniques for learning stem cells and their supportive stromal cells also are mentioned. The protocols stick with the profitable equipment in Molecular Medicineв„ў sequence layout, each supplying step by step laboratory directions, an creation outlining the primary at the back of the method, lists of the required gear and reagents, and tips about troubleshooting and heading off recognized pitfalls.

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Orkin, S. , and Bessler, M. (2001) GATA1-Cre mediates Piga gene inactivation in the erythroid/megakaryocytic lineage and leads to circulating red cells with a partial deficiency in glycosyl phosphatidylinositol-linked proteins (paroxysmal nocturnal hemoglobinuria type II cells) Blood 98, 2248–2255. 46. , and Fassler, R. (2001) Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice. J. Cell Sci. 114, 671–676. 47. 47 Emambokus, N. R. and Frampton, J.

Hammer, R. , Williams, S. , Richardson, J. , and Yanagisawa, M. (2001) Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Dev. Biol. 230, 230–242. 55. , and Busslinger, M. (2001) Pax5/BSAP maintains the identity of B cells in late B lymphopoiesis. Immunity 14, 779–790. 56. 56 Wilson, T. , Cowdery, H. , and Hertzog, P. J. (2002) A human CD2 minigene directs CRE-mediated recombination in T cells in vivo. Genesis 33, 181–184. 57. 57 Cheung, A. , Hande, M. , Tsao, M.

Sci. USA 96, 5037–5042. 45. , Orkin, S. , and Bessler, M. (2001) GATA1-Cre mediates Piga gene inactivation in the erythroid/megakaryocytic lineage and leads to circulating red cells with a partial deficiency in glycosyl phosphatidylinositol-linked proteins (paroxysmal nocturnal hemoglobinuria type II cells) Blood 98, 2248–2255. 46. , and Fassler, R. (2001) Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice. J. Cell Sci. 114, 671–676. 47. 47 Emambokus, N.

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