Download Blood Cells A Practical Guide, 5 edition by Barbara J. Bain PDF

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By Barbara J. Bain

- permits either the haematologist and laboratory scientist to spot blood telephone positive factors, from the commonest to the extra obscure
- presents crucial details on equipment of assortment, blood movie coaching and marking, including the foundations of guide and automatic blood counts
- thoroughly revised and up-to-date, incorporating a lot newly released details: now comprises suggestion on extra exams while a particular prognosis is suspected
- four hundred top of the range pictures to help with blood mobilephone identification
- Highlights the aim and medical relevance of haematology laboratory checks all through

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Extra resources for Blood Cells A Practical Guide, 5 edition

Sample text

The ICSH recommends that the differential leucocyte count be expressed in absolute numbers [30] and this is strongly recommended. A differential count carried out by a human observer using a microscope is referred to as a manual differential count. It is usually performed on a wedge‐spread ilm, the ilm being prepared either manually or with a mechanical ilm spreader. Automated differential counts are now generally performed by low cytometry as part of a full blood count (FBC), differentiation between categories being based on the physical characteristics of the cells and sometimes on their biochemical characteristics.

The use of platelet‐rich plasma may be preferred if the platelet count is low. When the method leaves platelets intact, large platelets can be distinguished from small red cells by the platelet’s shape, which may be oval rather than round, and by its irregular outline, with ine projections sometimes being visible. Use of ammonium oxalate, which lyses red cells, as a diluent produces a higher and more accurate count than use of formol‐citrate, which leaves red cells intact [24]. Platelet counts are best performed on anticoagulated venous blood obtained by a clean venepuncture.

Misidentiication of cells and unidentiiable cells Inaccuracy due to misidentiication of cells is usually not great when differential counts are performed by experienced laboratory workers on high‐quality blood ilms. An exception to this is the differentiation between band forms and segmented neutrophils. Criteria for making this distinction differ between laboratories, and there is also inconsistency in the application of the criteria within a laboratory because of an element of subjectivity. Occasionally it is also dificult to distinguish a monocyte from a large lymphocyte or a degranulated basophil from a neutrophil.

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