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By Shaker A. Mousa

1This selection of overview articles highlights the most recent improvement of antithrombotics and gives confirmed experimental tools for the extra improvement of latest and more suitable anticoagulants. one of the state of the art advancements reviewed are the unconventional utilization of low molecular weight heparins, such antithrombin brokers because the hirudin, and such antiplatelet medicines because the GPIIb/IIIa inhibitors and ADP receptor antagonists. extra options mentioned comprise aspirin and clopidogrel, the extended use of polytherapeutic techniques, antiproteases (factors IIa, Xa, and VIIa), tissue issue focusing on, platelet receptor focusing on, and antithrombin III modulation.

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McBride, S. , Trouton, T. , and Wilson, C. (2002) Randomized comparison of enoxaparin with unfractionated heparin following fibrinolytic therapy for acute myocardial infarction. Eur. Heart J. 23, 627–632. 35. Direct Thrombin Inhibitor Collaboration Investigators (2002) Direct thrombin inhibitors in acute coronary syndromes: principal results of a meta-analysis based on individual patient’s data. Lancet 359, 294–302. CH03,21-34,14pgs 9/5/03 11:01 AM Page 21 3 Evaluation of Platelet Antagonists in In Vitro Flow Models of Thrombosis Owen J.

2. Allow platelets to bind to APES-treated cover slip in a humid environment at 37°C for 30 min. CH03,21-34,14pgs 9/5/03 11:01 AM Page 30 30 McCarty et al. 3. Monocytic THP-1 Cell-Platelet Adhesion Assay 1. Assemble the platelet-coated cover slip on a parallel-plate flow chamber that is then mounted on the stage of an inverted microscope equipped with a CCD camera connected to a VCR and TV monitor. 2. Perfuse the antiplatelet antagonist at the desirable concentration or vehicle (control) over surface-bound platelets, and incubate for 10 min.

19. Abulencia, J. , McCarty, O. J. , Mousa, S. , and Konstantopoulos, K. (2001) Comparative antiplatelet efficacy of a novel, nonpep- CH03,21-34,14pgs 34 20. 21. 22. 23. 24. 9/5/03 11:01 AM Page 34 McCarty et al. tide GPIIb/IIIa antagonist (XV454) and Abciximab (c7E3) in Flow Models of Thrombosis. Arterioscler. Thromb. Vasc. Biol. 21, 149–156. Turner, N. , Moake, J. , Kamat, S. , Schafer, A. , Kleiman, N. , and McIntire, L. V. (1995) Comparative real-time effects on platelet adhesion and aggregation under flowing conditions of in vivo aspirin, heparin, and monoclonal antibody fragment against glycoprotein IIb-IIIa.

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